Plasmid
Part:BBa_I51000:Design
Designed by: Reshma Shetty Group: (2006-03-13)
BioBrick base vector, as originally designed
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1327
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1327
Illegal NheI site found at 177
Illegal NheI site found at 1126
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 1333 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1327
Illegal BglII site found at 1791 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1327
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1327
Illegal XbaI site found at 1342
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBa_I51000 is the original design of the BioBrick base vector BBa_I51020. Please see BBa_I51020 for complete design notes on the BioBrick base vector.